Person looking in a microscope.

About the Roballo Lab of Peripheral Nerve Repair 

My laboratory is focused on (1) developing new methods that will determine the best nerve graft for each peripheral nerve injury case, (2) investigating the potential of cell derived extracellular vesicle populations and cargo (miRNAs, biomarkers) that could improve current regenerative therapies targeting individualized care after central and peripheral nervous system injury, and (3) understanding the relationship between glial cells, neurons, and immunomodulatory cells (immune cells and stem cells) in the success and failure of tissue regeneration.

 

Principal Investigator

Headshot of Kelly Roballo.

Kelly C. S. Roballo, DVM, PhD

Assistant Professor of Neuroscience and Physiology
Edward Via College of Osteopathic Medicine
Bluefield University
kroballo@vt.vcom.edu

I am an Assistant Professor of Neuroscience and Physiology at VCOM, with a research laboratory at COHR. My formal educational training was in Veterinary Medicine (D.V.M), Animal Bioscience and Neuroscience (Ph.D.) both at University of Sao Paulo, Brazil, and Neuroscience and Immunology (Post doc) at University of Wyoming.

My background and my skillsets are wide-ranged, and over the past 12 years I have expanded my knowledge to new areas of biology, biomedicine, and bioengineering.

I am an early-stage investigator currently most active in the study of peripheral nerve and spinal cord physiology and injury, axon organization, and the development of computational methods for analyzing and interpreting peripheral nerve physiologic and pathologic mechanisms.

When not in the lab or teaching, I enjoy cooking, skiing, hiking, and travelling.

Current Lab Members

 

Headshot of Zachary Simmons.

Zachary T. Simmons

Edward Via College of Osteopathic Medicine- OMS II
D.O. Candidate, VCOM-VA

I am currently a 3rd year Medical Student at VCOM-VA. I have a bachelor’s degree in human nutrition, food and exercise from Virginia Tech and have since dedicated my time towards the field of medicine; more specifically the desire to become a surgeon. I am the President of my classes Surgical Organization and lead many events related to this while also participating in community outreach. In the lab, my team’s project is focused on the delivery of proteins using extracellular vesicles with the final goal of delivering a platelet protein to a specific cell. This will be further used in the field of neuroscience and peripheral nerve repair.

When not in lab or medical school I enjoy cooking, working on my physical fitness and snowboarding.



Headshot of Carey Hung.

Carey Hung

Edward Via College of Osteopathic Medicine- OMS II
D.O. Candidate, VCOM-VA
BS Biology, George Mason University
chung@vt.vcom.edu

I am currently a DO student at Edward Via College of Osteopathic Medicine. I joined Dr. Roballo's lab in 2022, and I'm working on a project that looks into remyelination of nerve transplants in rodent models. As of now, I'm interested in anesthesiology and radiology as possible specialties to go into.

When I'm not studying or in the lab, I like to hike, read books, and bake!



Headshot of Gabriella Kayal.

Gabriella Kayal

Edward Via College of Osteopathic Medicine- OMS II
D.O. Candidate, VCOM-VA
gkayal@vt.vcom.edu

I am a 3rd medical student at Edward Via College of Osteopathic Medicine (VCOM) and am working in Dr. Roballo's peripheral nerve regeneration lab. Other colleagues and I are currently examining and quantifying the remyelination and fusion of transplant nerve axons to donor nerves in rodent models. I plan to apply the experience and knowledge gained through this research, my Neuroscience background, and medical education to my future goal of becoming a neurologist, specializing in either spinal cord injury rehabilitation or movement disorders. The beauty of the nervous system is its role in connecting our mind, body, and spirit, which encapsulates the essence of osteopathy viewing the individual as one harmonious unit. Thus, contributing to a project, such as this, that can impact many lives in the future is very humbling.



Headshot of Reilly Ellis.

Reilly Ellis

Edward Via College of Osteopathic Medicine- OMS II
D.O. Candidate, VCOM-VA

I am a 3rd medical student at Edward Via College of Osteopathic Medicine – Virginia Campus. I joined Dr. Roballo’s lab in 2022. I am currently working on a project that analyzes the remyelination of nerve transplants in rodent models. The specialties I am interested in are neurology and anesthesiology.

In my free time, I enjoy baking, reading, and watching movies.



Headshot of Grey Fritz.

Grey Fritz

Edward Via College of Osteopathic Medicine- OMS II
D.O. Candidate, VCOM-VA

I am a 3rd medical student in the Roballo lab. I joined the lab in Spring 2022 because of my interest in neurology and neuroscience. My current project is researching how cell priming influences Schwann cell exosome cell uptake in endothelial cells. The overarching aim of the project is to enhance the regenerative capabilities of peripheral nerve cells which could be applied to peripheral nerve injury.

Outside of lab, I enjoy dancing, paddleboarding, watching Netflix, and reading.



Headshot of Malek Zahed.

Malek Zahed

Edward Via College of Osteopathic Medicine- OMS II
D.O. Candidate, VCOM-VA

I am a 4th year medical student at VCOM-VA working in Dr. Roballo’s lab. The project I am involved in spans the fields of peripheral nerve analysis, injury, and repair. I am developing an image processing program that will analyze and recognize various aspects of the intraneural structures in hope of creating a more targeted and patient-specific therapeutic approach and improving the outcomes of peripheral nerve injuries. I am originally from Tripoli, Lebanon and have an interest in pursuing neurological surgery.



Headshot of Taylor Wynne.

Taylor Wynne

Edward Via College of Osteopathic Medicine- OMS II
D.O. Candidate, VCOM-VA
twynne@vt.vcom.edu

I am a 3rd VCOM student in Dr. Roballo's lab. I have a strong interest in neurology and my research interests are in neurodegenerative diseases and regenerative medicine. My current research is in peripheral nerve damage, and I am studying the effects of neuronal cell priming on endothelial extracellular vesicle uptake and protein transport. The ultimate goal of this project is to investigate potential therapeutic advancements for peripheral nerve transplant and regeneration.



Headshot of Alex Pam.

Alex Pam

Edward Via College of Osteopathic Medicine- OMS II
D.O. Candidate, VCOM-VA

I am a DO candidate in Dr. Roballo’s lab, and I am broadly interested in the landscape of neurology. I completed her bachelor’s degree in Biology at Virginia Tech in 2020. Prior to joining the lab, I worked at the Virginia Spine Institute with the Pain Management team working with the neurologist specializing in neuromusculoskeletal disorders. I hope to eventually break into the field of neurology.

Outside of studying in medical school, I enjoy playing sports along with watching movies.



Headshot of Hasti Izadpanah.

Hasti Izadpanah

Edward Via College of Osteopathic Medicine- OMS IV
D.O. Candidate, VCOM-VA

My name is Hasti Izadpanah. I love working on neurology related research. When I was an undergrad, I worked on Neuromuscular research and in medical school I focused on nerve related research, nowadays I am working on my path to become neurologist.



Alumni

  • Laurel Romano, Bluefield University/VCOM MABS Student
  • Kim Nguyen, Edward Via College of Osteopathic Medicine- OMS II
  • Alekhya Peruri, Edward Via College of Osteopathic Medicine- OMS III
  • Mason Yount, Edward Via College of Osteopathic Medicine- OMS III
  • Alexandra Morgan, Edward Via College of Osteopathic Medicine- OMS III
  • Valarie Zanin, Undergraduate Student
Research team standing in the lab, all wearing white coats.

 

Kelly C. Santos Roballo, DVM, Ph.D.

Assistant Professor of Physiology and Neuroscience
Department of Biomedical Affairs
Edward Via College of Osteopathic Medicine
2265 Kraft Dr SW, Room 341 and 1410 Prices Fork, 441, Room 109
Blacksburg, VA 24060    
Phone: 540-231-2702
kroballo@vt.vcom.edu

 

PhD, Animal Bioscience, 2017
College of Veterinary Medicine
University of São Paulo
Pirassununga, SP, Brazil

DVM, 2015
College of Veterinary Medicine
University of São Paulo
Pirassununga, SP, Brazil

2021-present
Assistant Professor of Physiology and Neuroscience
Edward Via College of Osteopathic Medicine (VCOM)
Blacksburg, VA

2021-present
Research Assistant Professor
Department of Biomedical Sciences and Pathobiology
Virginia-Maryland College of Veterinary Medicine
Virginia Tech
Blacksburg, VA

2017-2021
Instructor and Post doctorate
School of Pharmacy
University of Wyoming
Laramie, WY

2015-2017
Teaching Assistant and PhD Student
College of Veterinary Medicine
University of São Paulo
Pirassununga, SP, Brazil

2012-2013
Student Intern
Department of Animal Sciences
University of Illinois
Champaign and Urbana, IL

  • 2022-present: Early Career Faculty Certificate Program, VCOM (in progress)
  • 2021: Biosafety training, Virginia Tech
  • 2019: Diversity, Equity & Inclusion Certification Program
  • 2019: Establishing Credibility as a Speaker
  • 2017-2019: Aseptic Surgery, Lab Animal Research, Basic Biosafety Training, Biosafety Complete Training Series, Large animals Research.
  • 2023-present: Peripheral Nerve Society
  • 2023-present: Sigma Xi
  • 2021-present: Society for Neuroscience
  • 2020-present: 500 Women Scientists
  • 2019-present: International Society for Extracellular Vesicles
  • 2017-present: TERMIS
  • 2016: University of São Paulo – College of Veterinary Medicine.  Awarded an honorable mention as the best PhD Project.
  • Present: Adviser for 13 first year students
  • Present: Adviser for 11 second year students
  • 2021- present: research adviser of 28 students
  • 2021- present: academic adviser of 25 students a year
  • 2021-present: medical school committee interviewer
  • 2021- present: Poster judge for research day
  • 2023: Virginia Tech- COHR Activities Committee member
  • 2022: VCOM Research conference committee member
  • 2022: VCOM Surgical journal club research mentor
  • 2022: Development block 5 gastrointestinal curriculum update

Editorial Boards & Invited Reviewer

  • 2020-present: Journal of Extracellular Vesicles (JEV)—Editorial Board 
  • 2021-2022: Life: Special Issue "Cell Culture and Genome Modification Technologies: from Biology to Biomedical Applications"—Guest Editor

Study Sections & Invited Reviewer

  • 2022: Invited review: Initiation to evaluate project from the Agencia Nacional de Investigación e Innovación- from the funding María Viñas, Uruguay

Ad-hoc reviewer

  • Ad-hoc reviewer for many journals including: Journal of Tissue Engineering and Regenerative Medicine, Animal Reproduction, Microscopy Research and Technique, Anatomia, Histologia, Embriologia, PLOS ONE Journal, Pesquisa Veterinária Brasileira, Acta Cirurgica Brasileira, and several others.

Research Mentorship
Current advisee
Edward Via College of Osteopathic Medicine (VCOM)
Blacksburg, VA

DO with Distinction

  • 2022-present: Malek Zahed, OMS III
  • 2022-present: Virginia Fritz, OMS II
  • 2022-present: Zachary Simmons, OMS II
  • 2022-present: Taylor Wynne, OMS II
  • 2022-present: Gabriella Kayal, OMS II
  • 2022-present: Carey Hung, OMS II
  • 2022-present: Reilly Ellis, OMS II
  • 2021-present: Mason Yount, OMS III
  • 2021-present: Alexandra Morgan, OMS III
  • 2021-present: Alekhya Peruri, OMS III

DO without Distinction

  • 2022-present: Dylan Miller, OMS I
  • 2022-present: Josiah Thomas, OMS I
  • 2022-present: Michael Reymundi Pabon, OMS II
  • 2022-present: Ananya Seth, OMS II
  • 2022-present: Alex PHAM, OMS II
  • 2022-present: Bridget Mellon, OMS II
  • 2022-present: Alida D'Souza, OMS II
  • 2022-present: Haejung Shin, OMS II
  • 2022-present: Kim Nguyen, OMS II
  • 2021-present: Thais Sloop, OMS III
  • 2021-present: Hasti Izadpanah, OMS III

MABS Program

  • 2022-present:Laurel Alexandria Romano, MABS

Virginia Tech

  • 2022-present: Valarie Zanin, VT Undergraduate

School of Pharmacy
University of Wyoming
Laramie, WY

  • 2019-2021: Daniel Burns, PhD student—Scientific accomplishments and outcomes: published 2 papers and 2 conference abstracts.
  • 2017-2021: Osimanjiang Wupu, PhD candidate—Scientific accomplishments and outcomes: published 2 papers and 2 conference abstracts.
  • 2018-2021: Tanasem Muna, Master’s candidate—Scientific accomplishments and outcomes: published 1 conference abstracts.
  • 2018-2021: Huong Pham, PharmD candidate—Scientific accomplishments and outcomes: published 1 paper and 1 conference abstract, current in school rotation for her Pharmacy degree.
  • 2018-2020: Michele Kilpatrick, Undergraduate—Scientific accomplishments and outcomes: published 2 conference abstracts, now is applying for master’s degree in Pathology at University of Wyoming.
  • 2018-2020: Kaitlyn Vap, Undergraduate—Scientific accomplishments and outcomes: published 1 conference abstract, now is applying for Medical School.
  • 2018-2020: Carson Asher, Undergraduate—Scientific accomplishments and outcomes: published 2 conference abstracts, now is applying for Medical School.
  • 2017-2018: Subash Dhugana, PhD candidate—Scientific accomplishments and outcomes: published 1 paper, now is working as a research assistant at University of California LA.
  • 2017-2018: Hayden True, Undergraduate—Scientific accomplishments and outcomes: published 1 conference abstract, now at Medical School (WWAMI) at University of Wyoming.

College of Veterinary Medicine
University of São Paulo
Pirassununga, SP, Brazil

  • 2015-2017: Vitoria M. Pereira, Undergraduate—Scientific accomplishments and outcomes:  published 2 papers, still working the lab and now a PhD candidate at University of Wyoming.
  • 2015-2017: Leticia R. Padoveze, Undergraduate—Scientific accomplishments and outcomes: studied abroad at University of Illinois with my former advisor Dr. Wheeler, published 1 conference abstract, now is practicing Veterinary Medicine.
  • 2016-2017: Felipe Ros, Undergraduate—Scientific accomplishments and outcomes: published 1 article, now master’s degree candidate at University of São Paulo.
  • 2016-2017: Maria Gabriela, Undergraduate—Scientific accomplishments and outcomes: published 1 conference abstract, now is practicing Veterinary Medicine.
  • 2016-2017: Patricia L. N. da Cunha, Undergraduate—Scientific accomplishments and outcomes: published 1 conference abstract, now is practicing Veterinary Medicine.
  • 2015-2016: Bárbara Rossi de Sousa, Undergraduate—Scientific accomplishments and outcomes: published 1 conference abstract, now is practicing Veterinary Medicine.

Peer-Reviewed Journal Articles (*corresponding author)

  1. Mason Y, Peruri, A, Morgan, A, Zahed, M, Fritz, V, Simmons, Z, Millard, J, Redden, DT, Reina, MA, Roballo KCS*. Morphology and morphometry of the human obturator nerve in males and females. Accepted Jan 2023, Anatomia, Histologia, Embryologia. 2023
  2. Allgood JE, Roballo KCS, Sparks BB, Bushman JS. The effects of graft source and orientation on outcomes after ablation of a branched peripheral nerve. Front Cell Neurosci. 2022 Nov 14;16:1055490. PMID: 36451654;
  3. Oliveira VC, Roballo KCS, Mariano Junior CG, Santos SPI, Meirelles FV, Bressan FF, Chiaratti MR, Tucker E, Davis E, Concordet JP, Ambrósio CE. HEK293T Cells with TFAM Disruption by CRISPR-Cas9 as a Model for Mitochondrial Regulation. Life 2022, 12, 22. PMID: 35054416
  4. Oliveira VC, Junior CGM, Belizário JE, Krieger JE, Bressan FF, Roballo KCS; Fantinato-Neto P, Meirelles FV, Chiaratti MR, Concordet JP, Ambrósio CE. Characterization of post-edited cells modified in the TFAM gene by CRISPR/Cas9 technology in bovine model. PlosOne, 2020, June. PMID: 32649732
  5. Maki AJ, Rabel RAC, Rubessa M, Ercolin ACM, Roballo KCS, Cooper JJ, Wheeler MB. Autologous Adipose-Derived Stem Cells, Platelet-Rich Plasma, and Fibrin Enhance Healing of Mandibular Bone Defects in Swine. International journal of regenerative medicine, 2020, Volume 3(2): 2-9. PMID: 34663761
  6. Osimanjiang W, Roballo KCS, Ito M, Antonopoulos A, Dell A, Haslam SM, Bushman J. Analysis of N- and O-glycans: Differential Glycan Expression After Rat Spinal Cord Injury. J Neurotrauma, 2020, Apr 22. PMID: 32316850
  7. Jones M, Nankervis B, Roballo KCS, Pham H, Bushman J, Coeshott C. A comparison of automated perfusion- and manual diffusion-based human regulatory T cell expansion and functionality using a soluble activator complex. Cell transplantation, 2020. PMID: 32662685
  8. Casals JB, Pieri NCG, Roballo KCS*, Bressan FF, Favaron F, Martins DS, Ambrosio CE. Pluripotent stem cells proliferation is associated with placentation in dogs. Anim Reprod, 2020. PMID: 33029216
  9. Roballo KCS, Burns D, Ghnenis AB, Osimanjiang W, Bushman JB. Long-term regeneration analysis after peroneal nerve injury using sheep as animal model. European Journal of Neuroscience, 2020. PMID: 32449561
  10. Pereira VM, Roballo KCS, Silva ANE, Cordeiro JF, Bressan TF, Muller AF, Ercolin ACM, Cirimbelli CF, Soares Hage MCFN, Carregaro AB and Ambrósio CE. The use of stem cells in reducing recovery time of distal limb fracture in seriema (Cariama Cristata). Journal of Avian Medicine and Surgery, 2020. PMID: 33099981
  11. Roballo KCS, da Silveira JC, Bressan FF, de Souza AF, Pereira VM, Porras JEP, Rós FA, Pulz LH, Strefezzi RF, Martins DDS, Meirelles FV, Ambrósio CE. Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration. Sci Rep. 2019, 1;9(1):11213. PMID: 31371742
  12. Roballo KCS, Dhungana S, Jiang Z, Oakey J, Bushman JS. Localized delivery of immunosuppressive regulatory T cells to peripheral nerve allografts promotes regeneration of branched segmental defects. Biomaterials. 2019, 209:1-9. PMID: 31022556
  13. de Oliveira VC, Moreira GSA, Bressan FF, Gomes Mariano Junior C, Roballo KCS, Charpentier M, Concordet JP, Meirelles FV, Ambrósio CE. Edition of TFAM gene by CRISPR/Cas9 technology in bovine model. PlosOne. 2019, 7;14 (3):e0213376. PMID: 30845180
  14. Schwarzbach SV, Melo CF, Xavier PLP, Roballo KCS, Cordeiro YG, Ambrósio CE, Fukumasu H, Carregaro AB Morphine, but not methadone, inhibits microsomal prostaglandin E synthase-1 and prostaglandin-endoperoxide synthase 2 in lipopolysaccharide-stimulated horse synoviocytes. Biochimie. 2019, 11;160:28-33. PMID: 30763639
  15. Roballo KCS, Souza AF, Lara VM, Pinheiro AO, Gomes IS, Karam RG, Martins DS, Machado LC, Ambrósio CE. Canine fetus immune system at late development. Anim Reprod, 2019, vol.16, n2, p.328-331. PMID: 33224294
  16. Roballo KCS, Bushman J. Evaluation of the host immune response and functional recovery in peripheral nerve autografts and allografts. Transpl Immunol. 2019, 5. pii: S0966-3274(18)30123-0. PMID: 30735701
  17. Pieri NCG, Mançanares ACF, de Souza AF, Fernandes H, Diaza AMG, Bressan FF, Roballo KCS, Casals JB, Binelli M, Ambrósio CE, Dos Santos Martins D. Xenotransplantation of canine spermatogonial stem cells (cSSCs) regulated by FSH promotes spermatogenesis in infertile mice. Stem Cell Res Ther. 2019, 20;10(1):135. PMID: 31109365  
  18. Zomer HD, Roballo KCS, Lessa TB, Bressan FF, Gonçalves NN, Meirelles FV, Trentin AG, Ambrósio CE. Distinct features of rabbit and human adipose derived mesenchymal stem cells: implications for biotechnology and translational research. Stem Cells Cloning. 2018, 23;11:43-54. PMID: 30425533
  19. de Souza AF, Pieri NCG, Roballo KCS, Bressan FF, Casals JB, Ambrósio CE, Perecin F, Martins DS. Dynamics of male canine germ cell development. Plos One. 2018, 28;13(2):e0193026. PMID: 29489867
  20. Gonçalves NJN, Bressan FF, Roballo KCS, Meirelles FV, Xavier PLP, Fukumasu H, Williams C, Breen M, Koh S, Sper R, Piedrahita J, Ambrósio CE. Generation of LIF-independent induced pluripotent stem cell from canine fetal fibroblasts. Theriogenology. 2017, 1;92:75-82. PMID: 28237347
  21. Machado LC, Roballo KCS, Cury FS, Ambrósio CE. Female reproductive system morphology of crabeating fox (Cerdocyon thous) and cryopreservation of genetic material for animal germplasm bank enrichment. Anat Histol Embryol. 2017, 46(6):539-546. PMID: 28913836
  22. Pieri N, Souza AF, Mançanares A, Roballo KCS, Casals JB, Ambrosio CE, Martins DS. Immunolocalization of proteins in the spermatogenesis process of canine. Reprod Domest Anim. 2017, 52 (2):170-176. PMID: 27774720
  23. Roballo KCS, Gonçalves NJ, Pieri NC, Souza AF, Andrade AF, Ambrósio CE. Regulation of neural stem cells by choroid plexus cells population. Neurosicence letters. 2016, 28;626:35-41. PMID: 27181512
  24. Morini AC, Pieri N, Roballo KCS, Martins DS, Ambrósio C, Morini Junior JC, Favaron PO, Minervino A, Pereira F, Miglino MA. Buffalo (Bubalus bubali) late embryo and foetus development. A morphologic analyses. Reprod Dom Anim. 2016, 51(4):509-14. PMID: 27272250
  25. Ercolin AC, Roballo KCS, Casals JB, Pieri NC, Souza AF, Barreto R da S, Bressan FF, Feitosa ML, Miglino MA, Meirelles FV, Ambrósio CE. Rabbit olfactory stem cells: isolation protocol and characterization. Acta Cir Bras. 2016, 31(1):59-66. PMID: 26840357
  26. Pieri N, Souza AF, Casals JB, Roballo KCS, Ambrósio CE, Martins DS. Comparative Development of Embryonic Age by Organogenesis in Domestic Dogs and Cats. Reprod Dom Anim. 2015,50, 625–631. PMID: 25990819
  27. Godoy Pieri NC, da Silva Santos PR, Roballo KCS, Flamini MA, Barbeito CG, Ambrosio CE, Miglino MA, Dos Santos Martins D. Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus). Anim Reprod Sci. 2014,149(3-4):316-24. PMID: 25069754
  28. Roballo KCS, Ercolin ACM, Casals JB, Pieri NCG, Barreto RSN, Illera MJ, Martins DS, Miglino MA, Ambrósio CE. Domestic Carnivore's Development: Detection of Oct-4, A Pluripotency Marker, in Pharyngeal Arches. Reprod Domest Anim. 2013, 48(3):e41-e43. PMID: 23379423
  29. Roballo KCS, Ercolin ACM, Bionaz M, Ambrosio CE, Wheeler MB. Porcine adipose-derived stem cells are induced toward neurogenic lineages by cell-to-cell interactions but not by soluble factors released by neurons isolated from adult and fetal brain. Reproduction Fertility and Development. 2012, 25(1):289.
  30. Abreu DK, Rodrigues EAF, Monteiro JM, Franciolli AL, Costola-Souza C, Roballo KCS, Ambrósio CE, Miglino MA. Estudo microscópico e macroscópico, com enfoque radiográfico e de alizarina, no desenvolvimento embrionário e fetal de gatos domésticos (Felis catus) em diferentes idades gestacionais. Pesq Vet Bras. 2011, 31(Supl.1):57-66. [Portuguese].
  31. Fadel L, Viana BR, Feitosa ML, Ercolin AC, Roballo KCS, Casals JB, Pieri NC, Meirelles FV, Martins Ddos S, Miglino MA, Ambrósio CE. Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep. Acta Cir Bras. 2011,26(4):267-273. PMID: 21808838

Book chapters (peer reviewed)

  1. Roballo KCS, Pereira VM, Ambrosio CE. Stem cell therapy in wild animals. Portuguese: Tratado de animais selvagens medicina veterinária, 3ª Ed.2022.
  2. Roballo KCS, Ambrosio CE, da Silveira JC. Protocol to Study the Role of Extracellular Vesicles During Induced Stem Cell Differentiation. Methods Mol Biol. 2021;2273:63-73. PMID: 33604844.
  3. Lara VM#, Roballo KCS#, Gotardo AT, Ambrósio CE. Flow Cytometry to Evaluate Potential Developmental Toxicants in the Embryonic Stem Cell. Methods Mol Biol. 2018;1797:165-175. PMID: 29896691. #equal contribution.

Review articles (peer reviewed)

  1. Peruri A, Morgan A, D'Souza A, Mellon B, Hung CW, Kayal G, Shin H, Nguyen K, Zahed M, Yount M, Ellis R, Wynne T, Fritz V, Simmons Z, Roballo KCS*. Pineal Gland from the Cell Culture to Animal Models: A Review. Life (Basel). 2022 Jul 15;12(7):1057. PMID: 35888145
  2. Bittner GD, Bushman JS, Ghergherehchi CL, Roballo KCS, Shores JT, Smith TA. Typical and atypical properties of peripheral nerve allografts enable novel strategies to repair segmental-loss injuries. J Neuroinflammation. 2022 Feb 28;19(1):60. doi: 10.1186/s12974-022-02395-0. PMID: 35227261
  3. Roballo KCS, Gigley JP,Smith TA, Bittner GD, Bushman JS. Review: Functional and Immunological Peculiarities of Peripheral Nerve Allografts. Neural Regeneration Research 17 (4):721-727. PMID: 34472457.
  4. Gomes IS, Oliveira VC, Pinheiro AO, Roballo KCS*, Araujo JSM, Veronezi JC, Martins DS and Ambrósio CE. Bone marrow stem cell applied in the canine veterinary clinics. Pesq. Vet. Bras. 2017, 37(10):1139-1145.
  5. Oliveira NA, Roballo KCS, Lisboa Neto AFS, Sandini TM, Santos AC, Martins DS, Ambrosio CE. Bioimpressão e produção de mini-órgãos com células tronco. Pesq. Vet. Bras. 2017, 37(9):1032-1039. [Portuguese].
  6. Mazzarella R, Garnica TK, Roballo KCS*, Ambrosio C.E. Células-tronco derivadas do epitélio olfatório: perspectivas terapêuticas na medicina veterinária. Pesq. Vet. Bras. 2016, 36(8):787-792 [Portuguese].
  7. Casals JB; Pieri NC, Feitose ML, Eroclin AC, Roballo KCS, Barreto RS, Bressan FF, Martins DS, Miglino MA, Ambrósio CE. The Use of Animal Models for Stroke Research: A Review. Comp Med. 2011, 61(4):305-313. PMID: 22330245

Published Abstracts (*corresponding author)

  1. Izadpanah H, Millard JA, Roballo KCS*. Evaluation of Obturator Nerve Fascicular Topography Using Landmark Morphometrics - a Pilot Study. FASEB Journal. Volume36, IssueS1.May 2022
  2. JA Allgood JA, Roballo KCS, Oakey J, Bushman J. Allografts in combination with local immune pression as a strategy to improve peripheral nerve regeneration. Journal of The Peripheral Nervous System 27, S8-S
  3. Roballo KCS, Dhugana S, Pham H, Burns D, Zhongliang J, Oakey J, Bushman J. Localized immune suppression achieved with drug and cell based strategies allow for full regeneration equivalent to nerve grafts in critical sized defects. Autumn Immunology Conference, Chicago IL. November 16-19th, 2018. (Poster and Oral presentation).
  4. Pieri NCG, Mançanares AFC, Souza AF, Roballo KCS, Casals JB, Ambrosio CE and Martins DS. Immunolocalization of Proteins in the Spermatogenesis Process of Canine. International Symposium on Canine and Feline Reproduction, 2016, Paris, France. Short Communication
  5. Ramos K L, Simoes LQ, Roballo KCS, Viana BR, Hage MCFNS, Sousa RLM, Grigoletto R, Aguair ML, Ribeiro MH, Doria RGS. O papel do exame ultrassonográfico no acompanhamento de abscesso cervical em equino. [The ultrasonography in the cervical abscess monitoring in equines.] Resumos da XIV Conferência Anual da Anual da Associação Brasileira dos Médicos Veterinários de Equídeos (ABRAVEQ) 2013;1:200-201. [Portuguese].
  6. Roballo KCS, Ambrósio CE, Bionaz M, Wheeler MB.  Porcine adipose-derived stem cells are induced toward neurogenic lineages by cell-to-cell interactions but not by soluble factors released by neurons isolated from adult and fetal brain. In: 39th IETS Annual Conference, 2013, Hannover. 39th IETS Annual Conference, 2012.
  7. Roballo KCS, Ercolin ACM, Casals JB, Moro MEG, Martins DS, Carvalho AF, Mançanares CF, Rosa RA, Miglino MA, Ambrósio CE. Análise macroscópica do aparelho reprodutor masculino do ouriço-cacheiro (Coendou villosus). In: XII Congresso ABRAVAS, 2009, Aguas de Lindoia. Anais do XII Congresso ABRAVAS., 2009.
  8. Ercolin ACM, Roballo KCS, Casals JB, Miglino MA, Martins DS, Moro MEG, Ambrósio CE. Análise macroscópica do aparelho reprodutor masculino de Seriema (Cariama cristata). In: XII Congresso ABRAVAS, 2009, Águas de lindóia. Anais do XII Congresso ABRAVAS, 2009.

Other Abstracts

  1. Chenchar A, Alexander BM, Roballo KCS, Zhang ZJ, Louiselle AE, Niemiec SM, Bardill J, Lehmann T, Zgheib C, Liechty KW, Nair S. Odanacatib, a Cathepsin K Inhibitor Accelerates Wound Healing in a Diabetic Porcine Model Anne. The Wound Healing Society, 2022.
  2. Yount M, Snyder J, Peruri A, Morgan A, Millard J, Roballo KCS*. Anatomical and biomechanical features of the donor-recipient allograft nerve: the rational and precise nerve matching approach in graft transplantation. Myelin 2022.
  3. Yount M, Peruri A, Morgan A, Millard J, Roballo KCS*. Obturator nerve macroanalysis and link between non-neural tissue within the nerve in association with Sex and laterality. Research day, February 2022.
  4. Snyder J, Millard J, Roballo KCS*. Femoral nerve macro analysis and its association with Sex. Research day, February 2022.
  5. Morgan A, Yount M, Peruri A, Millard J, Roballo KCS*. Obturator nerve macroanalysis and its association with laterality. Research day, February 2022.
  6. Peruri A, Yount M, Morgan A, Millard J, Roballo KCS*. Obturator nerve macro analysis and its association with sex. Research day, February 2022.
  7. Roballo KCS, Allgood J, Bushman J. The influence of allograft transection site on regeneration in a branched segmental defect. FRNG, Fort Collins CO, 2021. (Poster)
  8. Roballo KCS, Bushman J. Evaluation of the host immune response and functional recovery in peripheral nerve autografts and allografts. FRNG, Fort Collins CO, 2019. (Poster)
  9. Pham HL, Roballo KCS, J Bushman. Cryopreserved human regulatory T cells (hTregs) maintain their viability and immune suppression functionality as a therapeutic alternative to fresh hTregs. FRNG, Fort Collins CO, 2019. (Poster)
  10. Osimanjiang W, Roballo KCS, Ito M, Antonopoulos A, Dell A, Haslam SM, Bushman JS. Analysis of n- and o-glycans: differential glycan expression after rat spinal cord injury. FRNG, Fort Collins CO, 2019. (Poster)
  11. Asher C, Roballo KCS, Ghnenis AB, Burns D, Osimanjiang W, Bushman J. Long term regeneration analysis after peroneal nerve injury using sheep as animal model. FRNG, Fort Collins CO, 2019. (Poster)
  12. Kilpatrick M, Roballo KCS, Vap K, Jiang Z, Oakey J, Bushman J. Viability of regulatory t-cells in peripheral nerve grafts after microinjection delivery. FRNG, Fort Collins CO, 2019. (Poster)
  13. Bushman J, Roballo KCS, Zhang Z, Oakey J. Regeneration of Branched Peripheral Nerve Defects Using Allografts and Localized Immunosuppression. TERMIS- AM Annual Conference, Orlando, 2019. (Poster)
  14. Roballo KCS, Dhugana S, Pham H, Burns D, Zhongliang J, Oakey J, Bushman J. Developing New Delivery Methods for Localized Immunosuppression. 8th Annual International Symposium on Regenerative Rehabilitation in Charlottesville VA, 2019. (Oral and Poster Presentation)
  15. Roballo KCS, Dhugana S, Pham H, Burns D, Zhongliang J, Oakey J, Bushman J. Regeneration of complex Peripheral nerve defects using allografts treated with localized immunossupressive therapy.  Rocky Mountain Regional Neuroscience Conference, Denver CO. April 26th, 2019. (Poster)
  16. Roballo KCS, Dhugana S, Pham H, Burns D, Zhongliang J, Oakey J, Bushman J. Regeneration of complex Peripheral nerve defects using allografts treated with localized immunossupressive therapy.  College Heath Science Research Day, Laramie WY. March 28th, 2019. (Poster)
  17. Roballo KCS, Dhugana S, Pham H, Burns D, Zhongliang J, Oakey J, Bushman J. Regeneration of complex Peripheral nerve defects using allografts treated with localized immunossupressive therapy. FRNG, Fort Collins CO, 2018. (Poster)
  18. Roballo KCS, Dhugana S, Pham H, Burns D, Zhongliang J, Oakey J, Bushman J. Localized immune suppression achieved with drug and cell based strategies allow for full regeneration equivalent to nerve grafts in critical sized defects. Autumn Immunology Conference, Chicago IL, 2018. (Poster and Oral presentation)
  19. Roballo KCS, Souza AF, Silveira JC, Bressan FF, Martins DS, Ambrosio CE. Extracellular vesicles of neurons are involved in the neural differentiation of mesenchymal stem cells. Front Range Neuroscience 15th Annual Meeting, Fort Collins, CO, 2017. (Poster Presentation)
  20. Pieri NCG, Mançanares, ACF, Souza AF, Roballo KCS, Casals JB, Ambrosio CE, and Martins DS. Immunolocalization of Proteins in the Spermatogenesis Process of Canine. International Symposium on Canine and Feline Reproduction, Paris, France, 2016. (Poster presentation).
  21. Roballo KCS, Casals JB, Martins DS, Ambrósio CE. Pluripotent stem cells in domestic carnivores (Canis familiaris and Felis catus) placentae. International Congress of Animal Reproduction.  Le Vinci, Tours, France, 2016. (Oral Presentation)
  22. Roballo KCS, Souza AF, Pierri NCG, Martins DS, Ambrósio CE. Identification, establishment, culture, and characterization of progenitors choroid plexus cells using porcine as animal model. VIII Congresso Brasileiro e I Latino-americano de Células-Tronco e Terapia Celular, Foz do Iguaçu, Brazil, 2014. (Poster)
  23. Maki A, Rabell C, Ercolin A, Roballo KCS, Cooper J, Wheeler M. Autologous Adipose-Derived Stem Cells with Platelet-rich Plasma or Fibrin Enhance Healing of Porcine Mandibular Bone Defects. ORS 2014 Annual Meeting
  24.  Roballo KCS, Ercolin AC, Bionaz M, Ambrosio CE, Wheeler MB. Porcine adipose-derived stem cells are induced toward neurogenic lineages by cell-to-cell interactions but not by soluble factors released by neurons isolated from adult and fetal brain. 39th Annual Conference of the IETS (International Embryo Transfer Society), Hannover, Germany, 2013. (Poster)
  25. Roballo KCS, Ambrósio CE, Bionaz M, Wheeler MB. Células-tronco derivadas de tecido adiposo de suínos induzidas para linhagens neurogênica por interações célula-célula, e por fatores liberados por neurônios isolados do cérebro adulto e fetal utilizando o suíno como modelo animal. [Porcine adipose-derived stem cells are induced toward neurogenic lineages by cell-to-cell interactions but not by soluble factors released by neurons isolated from adult and fetal brain.] 21 Simpósio Internacional de Iniciação Científica da USP (SIICUSP); Universidade de São Paulo, 2013 [Portuguese]. (Oral Presentation)
  26. Souza AF, Pieri NCG, Casals JB, Roballo KCS, Ambrósio CE, Meirelles FV, Martins DS. Biological study canine embryonic germ cell. In: IV International Symposium on Animal Biology of Reproduction (ISABR), 2012. (Poster)
  27. Pieri NCG, Barreto RSN, Roballo KCS, Ambrósio CE, Meirelles FV, Martins DS. Relation of photoperiod with spermatogenic cycle in viscachas (Lagostomus maximus). In: IV International Symposium on Animal Biology of Reproduction (ISABR), 2012. (Poster)
  28. Roballo KCS, Miglino MA, Ercolin ACM, Casals JB, Martins DS, Meirelles FV, Ambrosio CE. Oct-4 Marker analysis of pluripotency of the pharingeal arches in dogs.  VI Congresso Brasileiro de Celulas Tronco e Terapia Celular, Salvador, Brazil, 2011. (Poster).
  29. Casals JB, Roballo KCS, Ercolin ACM, Issac JA, Pieri NCG, Miglino, MA Ambrósio CE. Induction of Stroke and Cell Therapy in Rabbit Model Using Fluoroscopy Examination as Auxilary. In: VI Congresso Brasileiro de Células Tronco e Terapia Celular, Salvador. Brazil, 2011. (Poster)
  30. Ercolin ACM, Miglino MA, Casals JB, Pieri NCG, Feitosa MLT, Bressan FF, Roballo KCS, Meirelles FV, Ambrósio CE. Primary culture of rabbit olfactory stem cells: isolation and characterization. In: Congresso Brasileiro de Celulas Tronco e Terapia Celular, Salvador Brazil, 2011. (Poster)
  31. Roballo KCS, Ercolin ACM, Casals JB, Ambrósio CE. Análise da pluripotencia dos Arcos Faríngeos em Gato Doméstico (Felis catus). [Analysis of pluripotency of Pharyngeal Arches in Domestic Cat (Felis catus).] 19 Simposio Internacional de Iniciação Científica da USP (SIICUSP); Universidade de São Paulo, 2011[Portuguese]. (Poster).
  32. Roballo KCS, Ercolin ACM, Casals JB, Martins DS, Miglino MA, Meirelles FV, Ambrósio CE. Analise do Potencial de Pluripotência dos Arcos faríngeos em carnívoros. [Analysis of pluripotency of pharyngeal arches in carnivores]. V Congresso Brasileiro de Celulas Tronco e Terapia Celular, 2010 (Gramado, Brazil) [Portuguese]. (Poster)
  33. Roballo KCS, Abreu DK, Rodrigues EAF, Ambrósio CE. Embryonary Development in Domestic Cats (Felis Catus). 18 Simposio Internacional de Iniciação Científica da USP (SIICUSP); Universidade de São Paulo, 2010. (Poster)
  34. Ercolin ACM, Casals JB, Roballo KCS, Feitosa MLT, Martins DS, Miglino M. A, Meirelles FV, Ambrósio CE. Comportamento das células-tronco de epitélio olfatório de cães transduzidas com os genes repórteres LacZ e GFP. In: Congresso Brasileiro de Celulas Tronco e Terapia Celular, 2010, Gramado. Brazil. (Poster)

Active

  • VCOM ICTAS, Roballo (PI) and VandeVord (Co-PI) 07/01/2022-06/30/2023, Investigating the regenerative postential do stem cells for use in blast spinal cord injury Role: PI, Total award $100,000

Complete

  • Travel grant- 9th Annual International Symposium on Regenerative Rehabilitation, $900. 2022
  • Travel grant- 8th Annual International Symposium on Regenerative Rehabilitation, $1,200. 2019        
  • Travel grant- University of Wyoming, for the expansion of collaboration at University of São Paulo, $2,000. 2018
  • Travel grant- International Congress on Animal Reproduction, €1,000. 2016
  • Ph.D. fellowship from State of São Paulo Research Support Foundation, ~R$74,800. 2015   
  • Travel grant- Pro-International Scholarship from University of Sao Paulo, $2000. 2013
  • Scientific Initiation fellowship from State of São Paulo Research Support Foundation, ~R$13,000. 2013
  • Scientific Initiation fellowship from State of São Paulo Research Support Foundation, ~R$13,000. 2010

Two people in lab coats reviewing slides on the computer. Person in a lab coat looking at a vial of dark liquid. Person in a lab coat looking at a vial of dark liquid. Person working on an experiment behind protective glass. Person in a white coat doing an experiment behind glass. Person looking in a microscope. Person looking in a microscope. Research team standing in the lab, all wearing white coats.